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Fig. 2 | The overall workflow of the Deepometry procedure. Step 1 (not shown here) guides users through the installation of the software and packages required to run Deepometry (Python/MATLAB, option A or B) or installation of the <t>standalone</t> application (MATLAB, option C). The application of Deepometry to image data analysis starts with Step 2. Steps 2 and 3, 6–8 and 12 and 13 are preprocessing actions for the training set, validation set and testing set, respectively, served to transform raw input images to data types and shapes appropriate for deep learning operations. Steps 4 and 5 are model training actions (highlighted in red). Steps 9–11 and 14–16 are predicting mechanisms for annotated data (highlighted in cyan) and unannotated data (highlighted in purple), respectively. Steps 17–21 are used to extract deep learning feature embeddings for dimension reduction and data exploration.
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Fig. 2 | The overall workflow of the Deepometry procedure. Step 1 (not shown here) guides users through the installation of the software and packages required to run Deepometry (Python/MATLAB, option A or B) or installation of the <t>standalone</t> application (MATLAB, option C). The application of Deepometry to image data analysis starts with Step 2. Steps 2 and 3, 6–8 and 12 and 13 are preprocessing actions for the training set, validation set and testing set, respectively, served to transform raw input images to data types and shapes appropriate for deep learning operations. Steps 4 and 5 are model training actions (highlighted in red). Steps 9–11 and 14–16 are predicting mechanisms for annotated data (highlighted in cyan) and unannotated data (highlighted in purple), respectively. Steps 17–21 are used to extract deep learning feature embeddings for dimension reduction and data exploration.
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Fig. 2 | The overall workflow of the Deepometry procedure. Step 1 (not shown here) guides users through the installation of the software and packages required to run Deepometry (Python/MATLAB, option A or B) or installation of the <t>standalone</t> application (MATLAB, option C). The application of Deepometry to image data analysis starts with Step 2. Steps 2 and 3, 6–8 and 12 and 13 are preprocessing actions for the training set, validation set and testing set, respectively, served to transform raw input images to data types and shapes appropriate for deep learning operations. Steps 4 and 5 are model training actions (highlighted in red). Steps 9–11 and 14–16 are predicting mechanisms for annotated data (highlighted in cyan) and unannotated data (highlighted in purple), respectively. Steps 17–21 are used to extract deep learning feature embeddings for dimension reduction and data exploration.
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Fig. 2 | The overall workflow of the Deepometry procedure. Step 1 (not shown here) guides users through the installation of the software and packages required to run Deepometry (Python/MATLAB, option A or B) or installation of the <t>standalone</t> application (MATLAB, option C). The application of Deepometry to image data analysis starts with Step 2. Steps 2 and 3, 6–8 and 12 and 13 are preprocessing actions for the training set, validation set and testing set, respectively, served to transform raw input images to data types and shapes appropriate for deep learning operations. Steps 4 and 5 are model training actions (highlighted in red). Steps 9–11 and 14–16 are predicting mechanisms for annotated data (highlighted in cyan) and unannotated data (highlighted in purple), respectively. Steps 17–21 are used to extract deep learning feature embeddings for dimension reduction and data exploration.
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Fig. 2 | The overall workflow of the Deepometry procedure. Step 1 (not shown here) guides users through the installation of the software and packages required to run Deepometry (Python/MATLAB, option A or B) or installation of the <t>standalone</t> application (MATLAB, option C). The application of Deepometry to image data analysis starts with Step 2. Steps 2 and 3, 6–8 and 12 and 13 are preprocessing actions for the training set, validation set and testing set, respectively, served to transform raw input images to data types and shapes appropriate for deep learning operations. Steps 4 and 5 are model training actions (highlighted in red). Steps 9–11 and 14–16 are predicting mechanisms for annotated data (highlighted in cyan) and unannotated data (highlighted in purple), respectively. Steps 17–21 are used to extract deep learning feature embeddings for dimension reduction and data exploration.
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Fig. 2 | The overall workflow of the Deepometry procedure. Step 1 (not shown here) guides users through the installation of the software and packages required to run Deepometry (Python/MATLAB, option A or B) or installation of the <t>standalone</t> application (MATLAB, option C). The application of Deepometry to image data analysis starts with Step 2. Steps 2 and 3, 6–8 and 12 and 13 are preprocessing actions for the training set, validation set and testing set, respectively, served to transform raw input images to data types and shapes appropriate for deep learning operations. Steps 4 and 5 are model training actions (highlighted in red). Steps 9–11 and 14–16 are predicting mechanisms for annotated data (highlighted in cyan) and unannotated data (highlighted in purple), respectively. Steps 17–21 are used to extract deep learning feature embeddings for dimension reduction and data exploration.
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Fig. 2 | The overall workflow of the Deepometry procedure. Step 1 (not shown here) guides users through the installation of the software and packages required to run Deepometry (Python/MATLAB, option A or B) or installation of the <t>standalone</t> application (MATLAB, option C). The application of Deepometry to image data analysis starts with Step 2. Steps 2 and 3, 6–8 and 12 and 13 are preprocessing actions for the training set, validation set and testing set, respectively, served to transform raw input images to data types and shapes appropriate for deep learning operations. Steps 4 and 5 are model training actions (highlighted in red). Steps 9–11 and 14–16 are predicting mechanisms for annotated data (highlighted in cyan) and unannotated data (highlighted in purple), respectively. Steps 17–21 are used to extract deep learning feature embeddings for dimension reduction and data exploration.
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Image Search Results


Fig. 2 | The overall workflow of the Deepometry procedure. Step 1 (not shown here) guides users through the installation of the software and packages required to run Deepometry (Python/MATLAB, option A or B) or installation of the standalone application (MATLAB, option C). The application of Deepometry to image data analysis starts with Step 2. Steps 2 and 3, 6–8 and 12 and 13 are preprocessing actions for the training set, validation set and testing set, respectively, served to transform raw input images to data types and shapes appropriate for deep learning operations. Steps 4 and 5 are model training actions (highlighted in red). Steps 9–11 and 14–16 are predicting mechanisms for annotated data (highlighted in cyan) and unannotated data (highlighted in purple), respectively. Steps 17–21 are used to extract deep learning feature embeddings for dimension reduction and data exploration.

Journal: Nature Protocols

Article Title: Deepometry, a framework for applying supervised and weakly supervised deep learning to imaging cytometry

doi: 10.1038/s41596-021-00549-7

Figure Lengend Snippet: Fig. 2 | The overall workflow of the Deepometry procedure. Step 1 (not shown here) guides users through the installation of the software and packages required to run Deepometry (Python/MATLAB, option A or B) or installation of the standalone application (MATLAB, option C). The application of Deepometry to image data analysis starts with Step 2. Steps 2 and 3, 6–8 and 12 and 13 are preprocessing actions for the training set, validation set and testing set, respectively, served to transform raw input images to data types and shapes appropriate for deep learning operations. Steps 4 and 5 are model training actions (highlighted in red). Steps 9–11 and 14–16 are predicting mechanisms for annotated data (highlighted in cyan) and unannotated data (highlighted in purple), respectively. Steps 17–21 are used to extract deep learning feature embeddings for dimension reduction and data exploration.

Article Snippet: Essential packages for the Python environment (see more details and download sites in the installation guide in Supplementary Note 1): ● Python 3.6 ● Tensorflow-gpu 1.9.0 ● Keras 2.1.5 ● Numpy 1.18.1 ● Scipy 1.4.1 ● Keras-resnet 0.0.7 ● Java Development Kit 8.0/11.0 ● Python-bioformats 1.5.2 ● Jupyter notebook Essential packages for the MATLAB environment (see more details and download sites in the installation guide in Supplementary Note 2): ● Image processing toolbox ● Deep Learning Toolbox Standalone application (the details and download sites are given in the installation guide in Supplementary Note 3)

Techniques: Software, Biomarker Discovery